Palm Tocotrienol-Rich Fraction Protects Neonatal Rat Cardiomyocytes from H2o2-Induced Oxidative Damage

Oxidative stress plays an important role in the pathogenesis of heart disease. Tocotrienol-rich fraction (TRF) is antioxidant and that has potential to reduce risk This study determine protective effects palm TRF against H2O2-induced oxidative damage neonatal rat cardiomyocytes (NRCM). The NRCM were divided into control, treated with (10 µg/mL), H2O2 (0.5 mM) prior induction (pre-treatment). Cell viability was determined by MTS assay,while presence reactive oxygen species (ROS) using fluorescent dihydroethidium (DHE) 5-(and-6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) dye. Mitochondrial integrity cell death JC-1 Annexin V-FITC staining, respectively. Lactate dehydrogenase (LDH) superoxide dismutase (SOD) activity colorimetric assay kit. concentration from 0.5 5 mM reduced IC50 value used experiment. increased intensity carboxy-H2DCFDA DHE-stains; also green fluorescence J-monomers staining compared control group. LDH while SOD decreased Pre-treatment intensities DHE-stains, as well JC-1. Meanwhile, pre-treatment group but no changes recorded Palm protects reducing ROS production maintaining mitochondrial membrane thus death.